Hydroxyapatite phagocytosis by human polymorphonuclear leucocytes.
نویسندگان
چکیده
Hydroxyapatite crystals were incubated with human polymorphonuclear leucocytes and samples examined by light and electron microscopy after 3, 8, 30, and 120 minutes' incubation. Phagocytosis of crystals occurred at 3 minutes and increased with incubation. Degranulation of neutrophils, loss of cytoplasmic density, and cell necrosis were greatest in cells mixed with apatite and increased with time. Avid in vitro phagocytosis of hydroxyapatite crystals lends further support to the potential of these crystals as causes of human arthritis.
منابع مشابه
Interactions of Candida albicans yeast cells, germ tubes and hyphae with human polymorphonuclear leucocytes in vitro.
Suspensions of Candida albicans yeast cells, germ tubes and hyphae with biomass standardized by ATP measurement were compared for their relative susceptibilities to phagocytosis and intracellular killing by human polymorphonuclear leucocytes. All three forms were ingested to a similar extent, but significantly fewer yeast cells were killed intracellularly after ingestion than were filamentous f...
متن کاملDegranulation of Polymorphonuclear Leucocytes following Phagocytosis of Microorganisms
A marked reduction in numbers of cytoplasmic granules in rabbit and human polymorphonuclear leucocytes takes place following ingestion of various microorganisms or of a yeast cell wall preparation. The degranulation occurs within 30 minutes of phagocytosis, and is directly related to the quantity of material engulfed. White cells completely degranulated following phagocytosis of large numbers o...
متن کاملInteractions between Rabbit Polymorphonuclear Leucocytes and Staphylococci
A method has been described for the study in vitro of leucocyte-bacteria interactions which permits the simultaneous evaluation of both phagocytosis and intracellular bacterial inactivation. Employing this technique, the fate and localization of staphylococci in homogeneous suspensions of rabbit polymorphonuclear leucocytes have been studied. Coagulase-positive strains of S. aureus were not eff...
متن کاملPhagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leucocytes.
The phagocytosis and killing of 3H-thymidine-labelled Staphylococcus aureus by polymorphonuclear leucocytes (PMNs) and monocytes (MNs) obtained from 50 health donors were evaluated. In addition, extracellular factors that might influence phagocytosis and killing were studied. The method described gave highly reproducible results. No significant difference was observed in the phagocytic and kill...
متن کاملPhospholipid turnover during phagocytosis in human polymorphonuclear leucocytes.
We have previously observed that the phagocytosis of zymosan particles coated with complement by human polymorphonuclear leucocytes is accompanied by a time- and dose-dependent inhibition of phosphatidylcholine synthesis by transmethylation [García Gil, Alonso, Sánchez Crespo & Mato (1981) Biochem. Biophys. Res. Commun.101, 740-748]. The present studies show that phosphatidylcholine synthesis b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Annals of the rheumatic diseases
دوره 38 1 شماره
صفحات -
تاریخ انتشار 1979